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KMID : 0368420070500030383
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Journal of Plant Biology
2007 Volume.50 No. 3 p.383 ~ p.385
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Overexpression of the acidic c-terminus region of hvraf from barley confers enhanced cadmium tolerance in yeast
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Jung Jin-Wook
Kim Min-Kyun
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Abstract
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The barley ERF-type transcription factor, HvRAF, has an acidic C-terminus domain that contains a hemopexin domain signature-like sequence. As an initial study to investigate the functioning of this domain, we cloned the partialHvRAF gene(pHvRAF) corresponding to the domain into a yeast/E.coli shuttle vector, pYES2. We then introduced thepYES2::pHvRAF recombinant plasmid into yeastSaccharomyces cerevisiae strain INVSd ). Here, we report that yeast transformants harboring pYES2::pHvRAF show significant tolerance to cadmium stress.
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KEYWORD
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cadmium stress, ERF-type transcription factor, Hordeum vulgare, Saccharomyces cerevisiae
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